PCR is a molecular biology technique used to amplify specific DNA fragments, special DNA replication in vitro,In experiments such as the construction of expression vectors and the expression of target proteins, it is usually necessary to obtain a target gene. We can use genomic DNA or Total RNA as a template based on the known reference sequence you provide (published on the NCBI), get a gene for a region you need. 

II. Technological process

Primer design and synthesis

Extraction of genomic DNA/Total RNA

The target gene was obtained by PCR amplification using genomic DNA as template or by RT-PCR amplification using Total RNA as template. 

III. Contents and criteria for reporting

1. Experimental reports (including test methods, photos of pcr products, etc.), electronic version results (including experimental reports, sequencing color waveforms, sequencing directions, alkali motifs, restriction site maps, etc.), primers.

2. For cloning, provide plasmids containing the target gene and puncture bacteria.

IV.Fees and Service Cycles 

Rates and service cycles vary depending on the sample provided by the customer ,Please contact Customer Service Officer for details or refer to the signed cooperation contract 

V. Quality assurance

1. Because of the individual difference of materials and the mutation in PCR process, we don’t guarantee that the genes obtained are identical to the bases of the reference sequence. 

2. Since some genes are expressed in cloned or expressed vectors, the expression products inhibit the growth of host bacteria.Therefore, we don’t guarantee that all genes can be successfully cloned or subcloned.

VI. After-sales service

The company will conduct customer visits on a regular basis to improve our service quality. The terms and conditions are governed by the contract of cooperation concluded and the details will vary according to the content of the contract.